Careprost is designed for those who suffer from Hypotrichosis - it is a disease in which there is an insufficient growth of eyelashes. The drug stimulates the growth of eyelashes and makes them longer, thicker and darker. The result will be noticeable within a few weeks after the start of use. For maximum results, it will take 2 to 4 months of daily use. To maintain the effect, you need to apply the product 1-2 times a week. If you do not continue applying, the eyelashes will return to their original state after 1-2 months.
Bimatoprost sol 0.03 % with 2 mM sodium lactoferrin for 48 h at 30°C as previously reported by Liu and co-workers [14]. Briefly, mice were fasted overnight at 4°C, and blood (1 mL) was removed and serum fat were separated. Fasting mice then euthanized and adipose tissue was removed by trituration. Fat (2.1 g tissue) was transferred to two sterile plastic 4-mL Erlenmeyer tubes (Tris-Pierce). For lipid extraction from tissue samples, two tubes were used to allow for the removal of blood from mice. A total of 2.2 mL blood was added to each tube and centrifugal force was applied for 3 minutes at 5,000 rpm. The fat that was extracted immediately suspended in 50 µL of 0.15M NaCl. Fat free from generic for bimatoprost ophthalmic adipose was suspended in 5 µL of 50 mM Tris-EDTA buffer at pH 9.1. To extract plasma glucose from adipose samples, 3 mL of fatty tissue was mixed with 12 mL 10 mM HEPES and centrifuged for 10 minutes at 10,000 rpm. The sample was immediately transferred to 100 µL of 10 mM Tris-buffered saline and centrifuged again for 10 minutes at 10,000 rpm. Approximately 2–5 µL of a 0.1% Tween 20 solution was added and then centrifuged again for 4 minutes at 10,000 rpm. The supernatant containing Tween 20 solution was discarded from the sample. Blood plasma adipose tissue was collected with a syringe at 3 minute intervals (as previously described) and the supernatant was centrifuged for 5 minutes at 10,000 rpm. The supernatant containing plasma was then transferred to a new sterile glass tube. Samples were allowed
bimatoprost ophthalmic solution generic to clot for at least 5 minutes 4 °C, and then centrifuged at 10,000 rpm for 10 minutes. The serum was centrifuged to remove cell debris and then the plasma collected in new sterile glass tube containing 0.2 mM EDTA or NaCI buffer (pH 7.5) and centrifuged for 10 minutes at 10,000 rpm. Aliquots of the supernatants were used to determine plasma glucose concentration by RIA. Aliquots of the plasma glucose concentrations were diluted to the correct concentration in 0.2 mM EDTA and 0.05 M sodium acetate buffer, and this solution was used to calibrate RIA. Fat plasma (40 mg tissue) was weighed and then homogenized in an 18 g Eppendorf tube. The homogenates and tubes were then stored in a refrigerator at 4 °C.
Binding of lipophilic BIPs to human adipose tissue and mice adipocytes
All the samples of lipid fractions and mouse adipose tissue used in this study were obtained from The Netherlands Institute for Biomedical Research. The fat used during study was obtained from the National Ethical Committee of Netherlands. Fatty tissue and adipose samples were homogenized mixed for binding experiments as previously reported [11]. The protein samples were added to a total weight of 1.5 g using PIPES. After this, 3 g of lipophilic BIPs were added to the samples of sample a final concentration 2.5 µM. To this mixture, 2 g of lipophilic BIPs were added in a volume of 200 µL. tube and cap were used to prevent direct contact with the samples of fat.
In order to determine the lipophilicity of BIPs used here, 1×1–4 ml aliquots of the BIP so